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KMID : 1098420130210040247
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Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 4 p.247 ~ p.254
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Genetic Diversity of the Original Plant for Taraxaci Herba, Taraxacum spp. by the Analysis of AFL
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Kim Wook-Jin
Moon Byeong-Cheol
Ji Yun-Ui
Lee Young-Mi
Kim Ho-Kyoung
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Abstract
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Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages
(UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the
species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.
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KEYWORD
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Taraxacum, AFLP, PCA, Genetic Relationship, Germplasm
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